DOI: https://doi.org/10.14710/jnc.v11i1.31603

PENGARUH PEMBERIAN SARI EDAMAME (Glycine max (L.) Merrill) TERHADAP KADAR ASAM URAT TIKUS WISTAR JANTAN DIABETES

Departemen Ilmu Gizi, Fakultas Kedokteran, Universitas Diponegoro, Indonesia

Received: 16 Jul 2021; Published: 31 Jan 2022.

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Abstract

Latar Belakang: Beberapa penelitian menunjukkan terdapat peningkatan risiko hiperurisemia pada pasien diabetes melitus tipe 2. Faktor yang mempengaruhi hiperurismeia yaitu penurunan ekskresi asam urat dan peningkatan produksi asam urat. Asupan antioksidan dan purin merupakan faktor yang mempengaruhi produksi asam urat. Edamame berpotensi menurunkan risiko hiperurisemia karena mengandung isoflavon tinggi dan purin yang sangat rendah

Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh pemberian sari edamame terhadap kadar asam urat tikus wistar diabetes.

Metode: Penelitian ini merupakan penelitian true experimental dengan pre-post test control design.  Sampel penelitian ini adalah 24 ekor tikus wistar jantan yang dibagi dalam 4 kelompok dan masing-masing terdiri atas 6 ekor tikus [K (-); K (+); P1; P2]. Kelompok K (+), P1, dan P2 diinduksi Streptozotocin 45 mg/kgBB dan Nicotinamide 110 mg/kgBB . Kelompok P1 dan P2 diberikan intervensi sari edamame dengan dosis 1,8 ml/hari dan 3,6 ml/hari selama 28 hari. Pengambilan sampel darah melalui plexus retroorbitalis. Pemeriksaan kadar asam urat menggunakan metode FS TBHBA. Perbedaan kadar asam urat pre-post intervensi dianalisis menggunakan uji paired t test. Perbedaan antar kelompok dianalisis menggunakan uji Kruskal-Wallis dengan uji lanjut Mann-Whitney.

Hasil: Pemberian sari edamame selama 28 hari menunjukkan perbedaan kadar asam urat yang signifikan (p<0,05). Kelompok P1 dan P2 mengalami penurunan kadar asam urat signifikan sebesar 4,62+0,28 mg/dl dan 5,43+0,15 mg/dl. Kelompok P1 dan P2 memiliki perbedaan kadar asam urat yang signifikan dibandingkan kelompok K(-) dan K(+).

Simpulan:  Pemberian sari edamame dosis 1,8 ml/hari dan 3,6 ml/hari secara efektif menurunkan kadar asam urat tikus wistar diabetes.

Note: This article has supplementary file(s).

Fulltext View|Download |  Data Analysis
Data Analisis Berat Badan dan Kadar Asam Urat
Subject Asam Urat, Diabetes Melitus Tipe 2, Isoflavon, Sari Edamame
Type Data Analysis
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 Research Materials
Perhitungan Dosis Purin
Subject Asam Urat, Diabetes Melitus Tipe 2, Isoflavon, Sari Edamame
Type Research Materials
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 Keterangan Layak Etik
Ethical Exemption No. 74/EC/KEPK/2020
Subject Asam Urat, Diabetes Melitus Tipe 2, Isoflavon, Sari Edamame
Type Keterangan Layak Etik
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Keywords: Asam urat, Diabetes melitus tipe 2, Isoflavon, Sari edamame

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