skip to main content

Deteksi Enzim Peroksidase dari Lobak Putih (Raphanus sativus L.) Berdasarkan Kadar Proteinnya

*Mauly Nabia Susanto  -  Program Studi S-1 Teknologi Pangan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Indonesia

Citation Format:
Abstract

Enzim peroksidase telah banyak digunakan sebagai reagen di bidang farmasi, industri maupun pangan. Lobak putih (Raphanus sativus L.) merupakan tanaman yang dikenal dengan kandungan enzim peroksidase dan senyawa bioaktif lainnya yang baik untuk kesehatan, namun pemanfaatannya masih terbatas. Penelitian ini bertujuan untuk  mendeteksi enzim peroksidase dari lobak putih berdasarkan kadar proteinnya dengan teknik pertukaran ion. Fraksi hasil elusi diukur absorbannya pada spektrofotometer dengan panjang gelombang 280 nm. Jumlah fraksi yang didapat sebanyak 29 fraksi. Berdasarkan pengamatan absorban, maka didapat hasil bahwa fraksi dengan urutan ke-5 merupakan fraksi yang mempunyai nilai absorbansi terbesar dan perkiraan kadar protein sebesar 1,387%, melalui uji Bradford kadar protein yang diperoleh sebesar 0,152%. Penelitian ini telah berhasil untuk melakukan pengambilan enzim peroksidase dan dapat digunakan sebagai informasi mengenai penelitian lebih lanjut.

Peroxidase enzyme has been widely used as reagent in pharmacy, industrial, and also food. Radish (Raphanus sativus L.) is a plant that has been known to contain peroxidase and other bioactive compounds that are good for health, but its applications are still limited. This study aims to detect peroxidase enzymes contained in radish based on its protein content by ion exchange techniques. The elution fraction was measured by absorbance on a spectrophotometer with a wavelength of 280 nm. Total obtained fraction from elution was 29 fractions. Based on absorbance observation, it was found that fraction with the order of-5 is the fraction that has the greatest absorbance value which has protein content estimation of 1,387%, protein content obtained through Bradford test was 0,152%. This study has succeeded in conducting peroxidase enzyme taking and particularly useful for advanced research.

Fulltext View|Download
Keywords: lobak; enzim peroksidase; ekstraksi; kandungan protein; pertukaran ion

Article Metrics:

  1. Al-Baarri, A. N., M. Ogawa and S. Hayakawa. 2010. Scale-up studies on immobilization of lactoperoxidase using milk whey for producing antimicrobial agent. Journal of the Indonesian Tropical Animal Agriculture. 35: 185–191. DOI: 10.14710/jitaa.35.3.185-191
  2. Al-Baarri, A. N. 2016. Peroksidase Daun Tomat dan Aplikasinya untuk Antibakteri. Penerbit Indonesian Food Technologist, Semarang
  3. Al-baarri, A.N., M. Hayashi, M. Ogawa and S. Hayakawa. 2011. Effects of Mono-and Disaccharides on the Antimicrobial Activity of Bovine Lactoperoxidase System. Journal of Food Protection 74(1): 134–139. DOI: 10.4315/0362-028X.JFP-10-184
  4. Bódalo, A., J. L. Gómez, E. Gómez, J. Bastida and M. F. Máximo. 2006. Comparison of commercial peroxidases for removing phenol from water solutions. Chemosphere 63(4): 626–632. DOI: 10.1016/j.chemosphere.2005.08.007
  5. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quntities of protein utilizing the principle of protein-Dye binding. Anal. Biochem 72: 248-254. DOI: 10.1016/0003-2697(76)90527-3
  6. Diao, M., O. H. Kone, N. Ouedraogo, R. G. Bayili, I. H. N. Bassole, and H. Mamoudou. 2011. Comparison of peroxidase activities from Allium sativum, Ipomoea batatas, Raphanus sativus and Sorghum bicolor grown in Burkina Faso. African Journal of Biochemistry Research 5(4): 124-128. ISSN 1996-0778
  7. Fitriansyah, B., A. N. Al-Baarri dan A. M. Legowo. 2015. Konsentrasi Minimum Sistem Laktoperoksidase untuk Menekan Pertumbuhan Escherichia coli pada Susu Sapi Segar. J. Teknol. dan Industri Pangan 26(1): 100-108. DOI: 10.6066/jtip.2015.26.1.100
  8. Jiménez-Atiénzar, M., M. A. Pedreño, N. Caballero, J. Cabanes and F. García-Carmona. 2007. Characterization of polyphenol oxidase and peroxidase from peach mesocarp (Prunus persica L. cv. Babygold). J. Sci. Food Agric 87: 1682–1690. DOI: 10.1002/jsfa.2886
  9. Kumaunang, M. dan V. Kamu. 2011. Aktivitas enzim bromelin dari ekstrak kulit nenas (Anenas comosus). Jurnal Ilmiah Sains 11(2):198-201. DOI: https://doi.org/10.1234/jis.v11i2.207
  10. Karossi, A. T. dan S. Pudjiraharti. 2010. Isolasi enzim horseradish peroksidase (HRP) dari kultur sel daun Armoracia lapatifolia dengan cara fraksinasi menggunakan amonium sulfat. JKTI 12(1): 20-25
  11. Kempe, H., A. Axelsson, B. Nilsson and G. Zacchi. 1999. Simulation of chromatographic processes applied to separation of protein. Journal of Chromatography A 846(1-2): 1-12. DOI: 10.1016/S0021-9673(98)01079-6
  12. Motoyama, A., T. Xu, C. I. Ruse, J. A. Wohlschlegel and J. R. Yates. 2007. Anion and cation mixed-bed ion exchange for enhanced multidimensional separations of peptides and phosphopeptides. Analytical chemistry 79(10): 3623-3634. DOI: 10.1021/ac062292d
  13. Pudjiraharti and A. T. Karossi. 2009. Purification And Characterization Of White Radish (Raphanus Sativus L. Var Long White) Peroxidase From Cell Culture Extract. Jurnal Teknologi Indonesia 32(2):91-98. DOI: 10.14203/jti.v32i2.8
  14. Purwanto, M.G.M. 2014. Perbandingan analisa kadar protein terlarut dengan berbagai metode spektroskopi UV - Visible. Jurnal Ilmiah Sains dan Teknologi 7(2): 64 - 71. ISSN 0216-1540
  15. Rakhmawati, R., E. Anggarwulan and E. Retnaningtyas. 2009. Potency of Lobak (Raphanus sativus L. var. hortensis Back) as Anticancer and Antimicrobial Candidates. Biodiversitas 10(3):158-162. DOI: 10.13057/biodiv/d100310
  16. Rodriguez-Lopez, J. N., J.C. Espin, J. Tudela, V. Martinez, A. Cerda and Garcia-Canovas, F. 2000. Purification and Kinetic Characterization of Peroxidase from Tomato Cultivated under Different Salinity Conditions. Journal of Food Science 65(1): 15-19. DOI: 10.1021/jf9905774
  17. Rusdi, B., D. Mulyanti and M. Rodiyah. 2014. Characterization of Peroxidase Enzyme from Water Spinach (Ipomoea aquatica Forssk.) Fraction. Procedia Chemistry 13(170-176). DOI: 10.1016/j.proche.2014.12.022
  18. Shoeb, M. 2006. Anticancer agents from medicinal plants. Bangladesh Journal Pharmacology 1: 35-41. DOI: 10.3329/bjp.v1i2.486
  19. Utami, T., A. N. Al-Baarri dan A. M. Legowo. 2017. Pengambilan Enzim Peroksidase dari Daun Tomat dengan Menggunakan Teknik Pertukaran Ion. Jurnal Aplikasi Teknologi Pangan 6(2): 85-88. DOI: 10.17728/jatp.220
  20. Yu, L. L and Y. Sun. 2012. Trace adsorption of positively charged proteins onto Sepharose FF and Sepharose FF-based anion exchangers. Journal of Chromatography A 1253: 105–109. DOI: 10.1016/j.chroma.2012.07.004

Last update:

No citation recorded.

Last update:

No citation recorded.