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Deteksi Enzim Peroksidase dari Lobak Putih (Raphanus sativus L.) Berdasarkan Kadar Proteinnya

Mauly Nabia Susanto  -  Program Studi Teknologi Pangan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Indonesia
*Ahmad Ni'matullah Al-Baarri scopus  -  Program Studi Teknologi Pangan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Indonesia
Anang Mohamad Legowo  -  Program Studi Teknologi Pangan, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Indonesia
Received: 29 Mar 2018; Published: 5 Jan 2022.
Editor(s): Siti Susanti, Ph.D

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Abstract

Enzim peroksidase telah banyak digunakan sebagai reagen di bidang farmasi, industri maupun pangan. Lobak putih (Raphanus sativus L.) merupakan tanaman yang dikenal dengan kandungan enzim peroksidase dan senyawa bioaktif lainnya yang baik untuk kesehatan, namun pemanfaatannya masih terbatas. Penelitian ini bertujuan untuk  mendeteksi enzim peroksidase dari lobak putih berdasarkan kadar proteinnya dengan teknik pertukaran ion. Fraksi hasil elusi diukur absorbannya pada spektrofotometer dengan panjang gelombang 280 nm. Jumlah fraksi yang didapat sebanyak 29 fraksi. Berdasarkan pengamatan absorban, maka didapat hasil bahwa fraksi dengan urutan ke-5 merupakan fraksi yang mempunyai nilai absorbansi terbesar dan perkiraan kadar protein sebesar 1,387%, melalui uji Bradford kadar protein yang diperoleh sebesar 0,152%. Penelitian ini telah berhasil untuk melakukan pengambilan enzim peroksidase dan dapat digunakan sebagai informasi mengenai penelitian lebih lanjut.

Peroxidase enzyme has been widely used as reagent in pharmacy, industrial, and also food. Radish (Raphanus sativus L.) is a plant that has been known to contain peroxidase and other bioactive compounds that are good for health, but its applications are still limited. This study aims to detect peroxidase enzymes contained in radish based on its protein content by ion exchange techniques. The elution fraction was measured by absorbance on a spectrophotometer with a wavelength of 280 nm. Total obtained fraction from elution was 29 fractions. Based on absorbance observation, it was found that fraction with the order of-5 is the fraction that has the greatest absorbance value which has protein content estimation of 1,387%, protein content obtained through Bradford test was 0,152%. This study has succeeded in conducting peroxidase enzyme taking and particularly useful for advanced research.

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Keywords: lobak; enzim peroksidase; ekstraksi; kandungan protein; pertukaran ion

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