Black Sea Cucumber (H. atra) is a type of marine biota that has ecological and economic value in the waters of the Karimunjawa Marine National Park. The purpose of this study was to analyze the relationship length-weight, condition factor and analysis of differences between Holothuria atra (black sea cucumbers) that live in cultivation and tourism zones and those that live in rehabilitation zones. Sea cucumber sampling is conducted at noon in 3 stations of the cultivation and tourism zones, namely Menjangan Kecil, Menjangan Besar and the waters around Wisma Apung and 1 rehabilitation zone station in Alang-alang. Observation of sea cucumber samples is done by census. Total samples obtained in Alang-alang were 30 individuals with a range of length 13-43 cm, weight 49-514 g, sampel in Menjangan Kecil were 17 individuals with a range of length 9-26 cm, weight 20-260 g, sampel in Menjangan Besar were 5 individuals with a range of length 16-23 cm, weight 66-193 g, and last sampel in waters around Wisma Apung were 17 individuals with a range of length 14-28 cm weight 23-260 g. The growth of sea cucumbers in each waters shows a negative allometric growth pattern which means that the addition of the length is faster than the addition of the weight. Fulton’s condition factor values of black sea cucumber in Alang-alang 1.34, Menjangan Kecil 1.91, Menjangan Besar 1.89, and waters around Wisma Apung 1.37, shows that black sea cucumbers in the waters that many visitors are fatter than black sea cucumbers in the Alang-alang which deserted visitors, and the relative weight condition factors of Alang-alang 104.27, Menjangan Kecil 104.25, Menjangan Besar 105.59 and Wisma Apung 108.22 each of the waters above 100 indicates that these waters provide sufficient food surplus.
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