skip to main content


*Hendy Wijaya  -  Laboratorium Biomedik, Fakultas Farmasi, Universitas Katolik Widya Mandala Surabaya, Indonesia
Siti Surdijati  -  Laboratorium Kimia Klinik, Fakultas Farmasi, Universitas Katolik Widya Mandala Surabaya, Indonesia
Received: 28 Oct 2019; Published: 25 Apr 2020.

Citation Format:

Latar Belakang: Untuk mengatasi masalah obesitas ada banyak program modifikasi gaya hidup melalui pengaturan pola makan dan peningkatan aktivitas fisik. Di antara program diet dikenal diet rendah lemak serta derivatnya dan diet rendah karbohidrat. Beberapa penelitian menunjukkan bahwa efek positif diet rendah karbohidrat sebagian besar diperantarai oleh keton dalam darah. Selain dapat berfungsi sebagai sumber energi alternatif yang efisien, keton dapat berperan juga sebagai molekul sinyal yang dapat mempengaruhi metabolisme sel dan perilaku. Virgin Coconut Oil (VCO) adalah sumber asam lemak rantai sedang alamiah dengan kuantitas dan kualitas tinggi yang dapat diserap dan dimetabolisme di dalam liver dengan mudah dan cepat menjadi keton

Tujuan: Penelitian ini dilakukan untuk mengetahui efek suplementasi virgin coconut oil terhadap kadar glukosa darah, keton darah, asupan makanan, massa lemak viseral dan berat badan pada model obesitas yang diinduksi melalui diet tinggi lemak-sukrosa.

Metode: Tiga puluh dua subyek tikus wistar jantan yang sebelumnya sudah dibuat obesitas (indeks Lee >0,300) melalui diet tinggi lemak-sukrosa selama 20 minggu dibagi ke dalam empat kelompok. Kelompok (A) mendapat akuades per oral, kelompok (S) mendapat larutan sukrosa per oral, kelompok (VCO) mendapat VCO per oral, dan kelompok (CO) mendapat minyak jagung per oral selama 4 minggu, diberikan sebelum makan pada siklus malam tikus.

Hasil: Hasil penelitian menunjukkan tidak ada perbedaan bermakna pada kadar glukosa darah, massa lemak viseral dan berat badan antar kelompok subyek. Hasil penelitian menunjukkan bahwa pemberian virgin coconut oil dapat meningkatkan kadar keton darah dan menurunkan asupan makanan (p=0,023 dan p=0,000) dibandingkan dengan kelompok perlakuan lain.

Simpulan: Hasil penelitian menunjukkan bahwa suplementasi VCO sebelum makan dapat meningkatkan kadar keton darah dan menekan asupan makanan tikus obesitas yang diinduksi diet tinggi lemak-sukros. Namun, penurunan asupan makanan tampaknya tidak dipengaruhi oleh kadar keton dalam darah.

Note: This article has supplementary file(s).

Fulltext View|Download |  Research Instrument
Komite Etik
Type Research Instrument
  Download (4MB)    Indexing metadata
Keywords: Virgin coconut oil; asam lemak rantai sedang; badan keton; asupan makanan; obesitas

Article Metrics:

  1. Stanner S, Thompson R, and Buttriss J. Healthy Ageing: The Role Of Nutrition and Lifestyle. UK: Wiley-Blackwell, 2009
  2. Lin EA, Barlow GM, and Mathur R. The Clinician’s Guide to the Treatment of Obesity. 1st rev. ed. Youdim A, Editor. California: Springer. 2015. 19-42 p
  3. The GBD 2013 Obesity Collaboration. Global, Regional, and National Prevalence of Overweight and Obesity in Children and Adults 1980-2013: A Systematic Analysis. The Lancet. 2013; 384(9945): 766-781
  4. Moore BJ, and Pi-Sunyer X. Textbook of Obesity: Biological, Psychological and Cultural Influences. 1st rev. ed. Akabas S, Lederman SA, and Moore BJ, Editors. Singapore: Wiley-Blackwell. 2012. 5-41 p
  5. Cox PJ, Kirk T, Ashmore T, Wilerton K, Evans R, Smith A, Murray AJ, Stubbs B, West J, McLure SW, King MT, Dodd MS, Holloway C, Neubauer S, Drawer S, Veech RL, Griffin JL, and Clarke K. Nutritional ketosis alters fuels preference and thereby endurance performance in athletes. Cell Metabolism. 2016; 24: 1-13
  6. Puchalska P and Crawford PA. Multi-dimensional roles of ketone bodies in fuel metabolism, signaling, and therapeutics. Cell Metabolism. 2017; 25: 262-284
  7. Kesl SL, Poff AM, Ward NP, Fiorelli TN, Ari C, Putten AJV, Sherwood JW, Arnold P, and D’Agostino DP. Effects of exogenous ketone supplementation on blood ketone, glucose, triglyceride, and lipoprotein levels in sprague-dawley rats. Nutrition and Metabolism. 2016; 13(9): 1-15
  8. Clegg ME. Medium-chain triglycerides are advantageous in promoting weight Loss although not beneficial to exercise performance. International Journal of Food Sciences and Nutrition. 2010; 61(7): 653-679
  9. Narayanankutty A, Mukesh RK, Ayoob SK, Ramavarma SK, Suseela IM, Manalil JJ, Kuzhivelil BT, and Raghavamenon AC. Virgin coconut oil maintains redox status and improves glycemic conditions in high fructose fed tats. Journal of Food Science and Technology. 2016; 53(1): 895-901
  10. Akinnuga AM, Jeje SO, Bamidele O, and Sunday VE. Dietary consumption of virgin coconut oil ameliorates lipid profiles in diabetic rats. Physiology Journal. 2014; 2014: 1-5
  11. Liau KM, Lee YY, Chen CK, and Rasool AHG. An open-label pilot study to asses the efficacy and safety of virgin coconut oil in reducing visceral adiposity. International Scholarly Research Network Pharmacology. 2011; 2011: 1-7
  12. Valente FX, Cândido FG, Lopes LL, Dias DM, Carvalho SMDL, Pereira PF, and Bressan J. Effects of coconut oil consumption on energy metabolism, cardiometabolic risk markers, and appetitive responses in women with excess body fat. European Journal of Nutrition. 2017; 57(4): 1627-1637
  13. Ihwah A, Deoranto P, Wijana S, and Dewi IA. Comparative study between federer and gomez method for number of replication in complete randomized design using simulation: study of areca palm (areca catechu) as organic waste for producing handicraft paper. IOP Conf. Series: Earth and Environmental Science. 2018; 131: 1-6
  14. Damasceno DC, Sinzato YK, Bueno A, Dallaqua B, Lima PH, Calderon IMP, Rudge MVC, and Campos KE. Metabolix profile and genotoxicity in obese rats exposed to cigarette smoke. Obesity. 2013; 21: 1596-1601
  15. Chusyd DE, Wang D, Huffman DM, and Nagy TR. Relationship between rodent white adipose fat pads and human white adipose fat depots. Frontiers in Nutrition. 2016; 3(10): 1-12
  16. Novelli ELB, Diniz YS, Galhardi CM, Ebaid GMX, Rodrigues HG, Mani F, Fernandes A AH, Cicogna AC, and Filho JLVBN. Anthropometrical parameters and markers of obesity in rats. Laboratory Animals. 2007; 41: 111-119
  17. Rasool S, Geetha T, Broderick TL, and Babu JR. High fat with high sucrose diet leads to obesity and induces myodegeneration. Frontiers in Physiology. 2018; 9(1054): 1-10
  18. Togo J, Hu S, Li M, Niu C, and Speakman JR. Impact of dietary sucrose on adiposity and glucose homeostasis in c57bl/6j mice depends on mode of ingestion: liquid or solid. Molecular Metabolism. 2019; 27: 22-32
  19. Bielohuby M, Menhofer D, Kirchner H, Stoehr BJ, Müller TD, Stock P, Hempel M, Stemmer K, Pfluger PT, Kienzle E, Christ B, Tschöp MH, and Bidlingmaier M. Induction of ketosis in rats fed low-carbohydrate, high-fat diets depends on the relative abundance of dietary fat and protein. American Journal of Physiology, Endocrinology and Metabolism. 2011; 300(1): 65-76
  20. Maki KC, Lawless AL, Kelley KM, Kaden VN, Geiger CJ, and Dicklin MR. Corn oil improves the plasma lipoprotein lipid profile compared with extra-virgin olive oil consumption in men and women with elevated cholesterol: result from randomized controlled feeding trial. Journal of Clinical Lipidology. 2015; 9: 49-57
  21. Botham KM and Mayes, PA. Harper’s Illustrated Biochemistry. 30th rev. ed. Murray RK, Granner DK, and Rodwell VW, Editors. Boston: McGraw-Hill. 2015. 223-231p
  22. Kashiwaya Y, Pawlosky R, Markis W, King MT, Bergman C, Srivastava S, Murray A, Clarke K, and Veech RL. A ketone ester diet increases brain malonyl-coa and uncoupling proteins 4 and 5 while decreaseing food intake in the normal wistar rat. The Journal of Biological Chemistry. 2010; 285(34): 25950-25956
  23. Stubbs BJ, Cox PJ, Evans RD, Santer P, Miller JJ, Faull OK, Magor-Elliott S, Hiyama S, Stirling M and Clarke K. On The metabolism of exogenous ketones in human. Frontiers in Physiology. 2017; 8: 848
  24. Carreiro AL, Dhillon J, Gordon S, Jacobs AG, Higgins KA, McArthur BM, Redan BW, Rivera RL, Schimdt LR and Mattes RD. The macronutrients, appetite and energy intake. Annu Rev Nutr. 2016; 36: 73-103
  25. Azari EK, Leitner C, Jaggi T, Langhans W, and Mansouri A. Possible role of intestinal fatty acid oxidation in the eating-inhibitory effect of the PPAR- agonist Wy-14643 in high-fat diet fed rats. PLos One. 2013; 8(9): 1-12
  26. Hughes SD, Kanabus M, Anderson G, Hargreaves IP, Rutherford T, O’Donnell M, Cross JH, Rahman S, Eaton S, and Heales SJ. The ketogenic diet component decanoic acid increases mitochondrial citrate synthase and complex i activity in neuronal cells. Journal of Neurochemistry. 2014; 129(3): 426-433
  27. Agustin K, Khabbush A, Williams S, Eaton S, Orford M, Cross JH, Heales SJR, Walker MC, and Williams RSB. Mechanism of action for the medium-chain triglyceride ketogenic diet in neurological and metabolic disorders. The Lancet Neurology. 2018; 17(1): 84-93
  28. Maher T, Sampson A, Goslawska M, Pangua-Irigaray C, Shafat A, and Clegg ME. Food intake and satiety response after medium-chain triglycerides ingested as solid or liquid. Nutrients. 2019; 11(1638): 1-11
  29. Byrne NM, Sainsbury A, King NA, Hills AP and Wood RE. Intermittent energy restriction improves weight loss efficiency in obese men: the MATADOR study. International Journal of Obesity. 2018; 42(2): 129-138
  30. Blomain ES, Dirhan DA, Valentino MA, Kim GW, and Waldman SA. Mechanism of weight regain following weight loss. ISRN Obesity. 2013; 2013:210524

Last update:

No citation recorded.

Last update:

No citation recorded.