DOI: https://doi.org/10.14710/dmj.v13i3.43904

The Effect of Moringa Leaves Extract on Sperm Motility in Male Mice Exposed to Electromagnetic Radiation of Mobile Phone

Hasnaa Alyasi Sabrina  -  Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
Trianggoro Budisulistyo  -  Neurology Department, Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
Achmad Zulfa Juniarto  -  Medical Biology Department, Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
*Tun Paksi Sareharto orcid publons  -  Medical Biology Department, Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia

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Abstract

Background: Electromagnetic radiation can induce an increase in oxidative stress. The presence of oxidative stress can impact the structure of the plasma membrane of sperm cells, damage the structure of Deoxyribonucleic Acid (DNA), and accelerate the process of apoptosis, ultimately leading to a decline in sperm quality. This increase in oxidative stress can be prevented by substances that serve as antioxidants. Moringa plants, particularly the leaves, contain various substances that can act as antioxidants for the body. These include flavonoids, alkaloids, saponins, tannins, and terpenoids. The antioxidant content in Moringa leaves is believed to safeguard the process of spermatogenesis

Aim: To determine the impact of Moringa (Moringa oleifera) leaf extract on the motility of spermatozoa in BALB/C mice subjected to exposure to electromagnetic waves.

Methods: This study utilized design featuring a post-test-only control group design, dividing participants into four randomly assigned groups, namely a negative control group, a positive control group (exposure to electromagnetic waves), and 2 treatment groups (moringa leaf extract at dose of 100 mg/kg BW and 400mg/kg BW). Each group compromised 5 experimental animals, and the treatments were administered for duration of 30 days. Spermatozoa preparations were made for each group and observed under a microscope (400x). Data were analyzed by initially testing for normality using the Shapiro-Wilk test. If the data distribution was normal, the hypothesis examination involved conducting a One Way ANOVA followed by subsequent Post Hoc testing.

Results: The results of the one-way ANOVA test indicated differences between groups of mice (p<0.05). The post hoc test results further demonstrated that a dose of 400 mg/kg BW was the most effective in preventing a decrease in spermatozoa motility. This effect is believed to be atttributed to the presence of vitamin C, beta carotene, beta-sitosterol, flavonoids, and polyphenols.

Conclusion: There is an improvement in spermatozoa motility in BALB/C mice exposed to electromagnetic waves after the administration of Moringa leaf extract.

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Keywords: Electromagnetic waves, Moringa leaf extract, Spermatozoa motility

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Section: Original Articles
Language : EN
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