slot gacor slot gacor hari ini slot gacor 2025 demo slot pg slot gacor slot gacor
The Effect of Moringa Leaves Extract on Sperm Motility in Male Mice Exposed to Electromagnetic Radiation of Mobile Phone | Sabrina | Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal)

DOI: https://doi.org/10.14710/dmj.v13i3.43904

The Effect of Moringa Leaves Extract on Sperm Motility in Male Mice Exposed to Electromagnetic Radiation of Mobile Phone

Hasnaa Alyasi Sabrina  -  Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
Trianggoro Budisulistyo  -  Neurology Department, Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
Achmad Zulfa Juniarto  -  Medical Biology Department, Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia
*Tun Paksi Sareharto orcid publons  -  Medical Biology Department, Medical Faculty, Universitas Diponegoro, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia

Citation Format:
Abstract

Background: Electromagnetic radiation can induce an increase in oxidative stress. The presence of oxidative stress can impact the structure of the plasma membrane of sperm cells, damage the structure of Deoxyribonucleic Acid (DNA), and accelerate the process of apoptosis, ultimately leading to a decline in sperm quality. This increase in oxidative stress can be prevented by substances that serve as antioxidants. Moringa plants, particularly the leaves, contain various substances that can act as antioxidants for the body. These include flavonoids, alkaloids, saponins, tannins, and terpenoids. The antioxidant content in Moringa leaves is believed to safeguard the process of spermatogenesis

Aim: To determine the impact of Moringa (Moringa oleifera) leaf extract on the motility of spermatozoa in BALB/C mice subjected to exposure to electromagnetic waves.

Methods: This study utilized design featuring a post-test-only control group design, dividing participants into four randomly assigned groups, namely a negative control group, a positive control group (exposure to electromagnetic waves), and 2 treatment groups (moringa leaf extract at dose of 100 mg/kg BW and 400mg/kg BW). Each group compromised 5 experimental animals, and the treatments were administered for duration of 30 days. Spermatozoa preparations were made for each group and observed under a microscope (400x). Data were analyzed by initially testing for normality using the Shapiro-Wilk test. If the data distribution was normal, the hypothesis examination involved conducting a One Way ANOVA followed by subsequent Post Hoc testing.

Results: The results of the one-way ANOVA test indicated differences between groups of mice (p<0.05). The post hoc test results further demonstrated that a dose of 400 mg/kg BW was the most effective in preventing a decrease in spermatozoa motility. This effect is believed to be atttributed to the presence of vitamin C, beta carotene, beta-sitosterol, flavonoids, and polyphenols.

Conclusion: There is an improvement in spermatozoa motility in BALB/C mice exposed to electromagnetic waves after the administration of Moringa leaf extract.

Fulltext View|Download
Keywords: Electromagnetic waves, Moringa leaf extract, Spermatozoa motility

Article Metrics:

Article Info
Section: Original Articles
Language : EN
Recent articles
The Effect of Benson’s Relaxation Therapy on Stress Levels Among Medical Students Diponegoro University Who Were Working on a Thesis RELATIONSHIP BETWEEN CLIMATE VARIABLES AND DENGUE INCIDENCE IN WEST INDONESIA Evaluation of the Quantity and Quality of Antibiotic Use in Inpatient Pediatric Urinary Tract Infection (UTI) Patients at RSUD KRMT Wongsonegoro Semarang More recent articles
  1. Noveriyanti N. Faktor risiko infertilitas pada wanita usia subur (Studi di klinik fertilitas Rumah Sakit Islam Sultan Agung Semarang). Universitas Muhammadiyah Semarang; 2017. Google Scholar [Internet]. [cited 2021 Dec 28]. Available from: http://repository.unimus.ac.id/397/3/05.%20bab%202.pdf
  2. Hendarto H, Wiweko B, Santoso B, Harzif AK. Konsensus penanganan infertilitas. Himpunan endokrinologi reproduksi dan fertilitas Indonesia, Perkumpulan Obstetri dan Ginekologi Indonesia. 2019
  3. Hamada AJ, Singh A, Agarwal A. Cell phones and their impact on male fertility: Fact or fiction. The Open Reproductive Science Journal. 2011;5:125-37
  4. Walczak–Jedrzejowska R, Wolski JK, Slowikowska–Hilczer J. The role of oxidative stress and antioxidants in male fertility. Cent Eur J Urol. 2013;66(1):60
  5. Dja’afara AL, Wantouw B, Tendean L. Pengaruh pemberian kopi terhadap kualitas spermatozoa tikus wistar jantan (Rattus norvegicus) yang diberi paparan asap rokok. e-Biomedik. 2015;3(2):676-680
  6. Eveline E, Siregar TM, Sanny. Studi aktivitas antioksidan pada tomat (Lycopersicon esculentum) konvensional dan organik selama penyimpanan. Prosiding Seminar Nasional Sains dan Teknologi. 2014;1(1):22-8
  7. Asih IARA, Sudiarta IW, Suci AAW. Aktivitas antioksidan senyawa golongan flavonoid ekstrak etanol daging buah terong belanda (Solanum betaceum cav.). Jurnal Kimia. 2015;9(1):35-40
  8. Kurniasih E. Khasiat dan manfaat daun kelor : untuk penyembuhan berbagai penyakit. Penerbit Pustaka Baru Press Yogyakarta. 2020
  9. Kesari KK, Kumar S, Behari J. Effects of radiofrequency electromagnetic wave exposure from cellular phones on the reproductive pattern in male Wistar rats. Appl Biochem Biotechnol. 2011;164(4):546-59
  10. Houston BJ, Nixon B, King BV, De Iuliis GN, Aitken RJ. The effects of radiofrequency electromagnetic radiation on sperm function. Reproduction. 2016;152:R263-76
  11. Singh A, Agarwal A. The role of sperm chromatin integrity and DNA damage on male infertility. The Open Reproductive Science Journal. 2011;3:65-71
  12. Aitken RJ, Koppers AJ. Apoptosis and DNA damage in human spermatozoa. Asian J Androl. 2011;13(1):36-42

Last update:

No citation recorded.

Last update:

No citation recorded.