ANALGESIC EFFECT OF KUMIS KUCING (ORTHOPSIPHON STAMINEUS) IN MICE (MUS MUSCULUS) WITH WRITHING METHOD

Joana Anjelia Nai Da Cruz; Endang Mahati; Noor Wijayahadi; Yora Nindita

doi:10.14710/dmj.v15i4.51910

DOI: https://doi.org/10.14710/dmj.v15i4.51910

ANALGESIC EFFECT OF KUMIS KUCING (ORTHOPSIPHON STAMINEUS) IN MICE (MUS MUSCULUS) WITH WRITHING METHOD

Joana Anjelia Nai Da Cruz - Faculty of Medicine,Universitas Diponegoro,Semarang, Indonesia

Endang Mahati - Department of Pharmacology,Faculty of Medicine,Universitas Diponegoro,Semarang, Indonesia

Noor Wijayahadi - Department of Pharmacology,Faculty of Medicine,Universitas Diponegoro,Semarang, Indonesia

*Yora Nindita

- Department of Pharmacology,Faculty of Medicine,Universitas Diponegoro,Semarang, Indonesia

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

BibTex Citation Data :

@article{dmj51910,
    author = {Joana Da Cruz and Endang Mahati and Noor Wijayahadi and Yora Nindita},
    title = {ANALGESIC EFFECT OF KUMIS KUCING (ORTHOPSIPHON STAMINEUS) IN MICE (MUS MUSCULUS) WITH WRITHING METHOD},
    journal = {Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal)},
  volume = {15},
    number = {4},
    year = {2026},
    keywords = {Orthosiphon stamineus, analgetic, writhing reflex.},
    abstract = {  Background:  Pain is an unpleasant sensation that can range from mild to severe, causing discomfort and potentially disturbing daily activities. Therefore, pain management and treatment are necessary.  Kumis kucing  ( Orthosiphon stamineus ) is a traditional medicinal plant known to contain flavonoids with anti‑inflammatory and analgesic properties that can help alleviate pain.  Objective:  This study aimed to investigate the analgesic activity and determine the effective dose of  Orthosiphon stamineus  extract.  Methods:  The design of this study was a true experimental post‑test‑only randomized controlled group. Pain was induced using a 1% acetic acid intraperitoneal injection to evoke the writhing reflex in mice. The writhing reflex was observed every 10 minutes for 1 hour, and the data were analyzed using one‑way ANOVA followed by a post‑hoc test.  Results:  The results revealed significant differences in analgesic activity between the P1 and P3 groups, as well as between the P2 and P3 groups (p < 0.05), indicating that the  kumis kucing  extract at a dose of 0.24 mg/gBW effectively reduced pain, as demonstrated by a decreased total number of writhing reflexes. The pain protection percentage at the 0.24 mg/gBW dose was 48.75%.  Conclusion:   Kumis kucing  extract has significant analgesic activity, with the most effective dose being 0.24 mg/gBW. },
   issn = {2540-8844},   pages = {311--315}  doi = {10.14710/dmj.v15i4.51910},
    url = {https://ejournal3.undip.ac.id/index.php/medico/article/view/51910}
}

Citation Format:

Abstract

Background: Pain is an unpleasant sensation that can range from mild to severe, causing discomfort and potentially disturbing daily activities. Therefore, pain management and treatment are necessary. Kumis kucing (Orthosiphon stamineus) is a traditional medicinal plant known to contain flavonoids with anti‑inflammatory and analgesic properties that can help alleviate pain. Objective: This study aimed to investigate the analgesic activity and determine the effective dose of Orthosiphon stamineus extract. Methods: The design of this study was a true experimental post‑test‑only randomized controlled group. Pain was induced using a 1% acetic acid intraperitoneal injection to evoke the writhing reflex in mice. The writhing reflex was observed every 10 minutes for 1 hour, and the data were analyzed using one‑way ANOVA followed by a post‑hoc test. Results: The results revealed significant differences in analgesic activity between the P1 and P3 groups, as well as between the P2 and P3 groups (p < 0.05), indicating that the kumis kucing extract at a dose of 0.24 mg/gBW effectively reduced pain, as demonstrated by a decreased total number of writhing reflexes. The pain protection percentage at the 0.24 mg/gBW dose was 48.75%. Conclusion: Kumis kucing extract has significant analgesic activity, with the most effective dose being 0.24 mg/gBW.

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Keywords: Orthosiphon stamineus, analgetic, writhing reflex.

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Section: Original Articles

Language : EN

In Vol 15, No 4 (2026): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)

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