DOI: https://doi.org/10.14710/dmj.v13i6.46228

Effect of Giving Pineapple Juice (Ananas comosus) on The Viability of Wistar Rat Spermatozoa Those Exposed to Cigarette Smoke

*Donna Hermawati  -  Dept of Medical Biology and Biochemistry Faculty of Medicine Universitas Diponegoro, Indonesia
Galang Dwi Prakoso  -  Medical Study Program, Faculty of Medicine, Diponegoro University, Indonesia
Mahayu Dewi Ariani  -  Department of Medical Biology and Biochemistry, Faculty of Medicine, Diponegoro University, Indonesia
Achmad Zulfa Juniarto  -  Department of Medical Biology and Biochemistry, Faculty of Medicine, Diponegoro University, Indonesia

Citation Format:
Abstract

Background: Infertility affects 15% of couples worldwide, with 40-50% of cases attributed to unhealthy lifestyle factors in men, such as smoking, alcohol consumption, drug use, obesity,poordietaryhabits,coffeeintake,andpsychologicalstress.Smokinghasanegative impactonreproductivehealthandreducesthechancesofhavingchildren.Smokingleadsto oxidative stress, lowers antioxidant levels in the testes, thereby disrupting spermatozoa formation. The antioxidant content in pineapple is expected to prevent and repair damage caused by oxidative stress. Objective: To investigate the effects of pineapple juice administration on sperm viability in Wistar rats exposed to cigarette smoke. Methods: Anexperimentalstudywasconductedwithapost-testonlygroupdesigninvolving 25maleWistarratsdividedinto5groups:K(notreatment),K+(exposuretocigarettesmoke only),T1(exposuretocigarettesmokeand1mlofpineapplejuiceperday),T2(exposureto cigarettesmokeand2.5mlofpineapplejuiceperday),andT3(exposuretocigarettesmoke and 4 ml of pineapple juice per day). All groups received treatment for 28 days. Results: ThemeanpercentageofspermatozoaviabilityinWistarratswasK=90.00;K(+)= 76.80; T1 = 76.80; T2 = 89.20; T3 = 93.40. Significant differences were observed between groupsKandK+andT1,withrespectivep-valuesof0.005.Significantdifferenceswerealso found between groups K+ and T2 (p = 0.008) and T3 (p = 0.001), while no significant difference was observed in group T1 (p = 1.000). Furthermore, there were significant differencesbetweengroupsT1andT2(p=0.008)andT3(p=0.001).Finally,nosignificant difference was found between groups T2 and T3 (p = 0.331). Conclusion and Recommendations: Pineapple juice can prevent and inhibit spermatozoa damage caused by free radicals from cigarette smoke. Further research should measure oxidative stress, provide exposure to each rat, and use varying doses for optimal effectiveness

Fulltext View|Download
Keywords: Cigarette, Spermatozoa, Pineapple, Viability

Article Metrics:

Article Info
Section: Original Articles
Language : EN
Recent articles
The Effect of Coffee Consumption Pattern on The Incidence of Dyspepsia in Medical Faculty Students The Correlation between Sleep Quality and Acne vulgaris Incidence in Medical Students of Universitas Tarumanagara Effectiveness of Adding Strengthening Lower Extremity Exercise on Gait Speed of Hajj Pilgrims Who Receive Walking Aerobic Exercises A 31-Year-Old Female with Inferior Paraparesis Upper Motor Neuron Type due to Destruction of Thoracic 6-7 Vertebrae due to Tuberculous Spondylitis : A Case Report Multiple Decubitus Ulcers in Dm type II Patients with Autologous Plasma-Rich Platelet therapy : A Case Report Characteristics of Intracerebral Hemorrhage at Dr. Kariadi General Hospital: A Serial Case More recent articles
  1. Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: A review of literature. J Hum Reprod Sci. 2015;8(4):191–6
  2. Payne N. Fertility Network UK Survey on the Impact of Fertility Problems. 2016;
  3. Mostafa S, Sabra MM, Al-Harbi MS. An Influential Relationship of Seminal Fluid Microbial Infections and Infertility, Taif Region, KSA. In 2014
  4. Durairajanayagam D. Lifestyle causes of male infertility. Arab Journal of Urology. 2018 Mar;16(1):10–20
  5. Ou Z, Wen Q, Deng Y, Yu Y, Chen Z, Sun L. Cigarette smoking is associated with high levels of ferroptosis in seminal plasma and affects semen quality. Reprod Biol Endocrinol. 2020 Dec;18(1):55
  6. Caserta D, Bordi G, Di Segni N, D'Ambrosio A, Mallozzi M, Moscarini M. The influence of cigarette smoking on a population of infertile men and women. Arch Gynecol Obstet. 2013
  7. Apr;287(4):813–8
  8. Ma'arif MZ, Suradi S, Sugiarto S. Effect of giving red dragon fruit, red guava, and their combination on total antioxidant capacity and malondealdehyde levels in adolescent smokers. JGI. 2020 Dec 18;9(1):53–60
  9. Alfa N, Mustofa S, Irawati NAV. Lycopene, an exogenous antioxidant that is beneficial for male fertility
  10. Managerxot J, Werdhasari A. The Role of Antioxidants for Health. Indonesian Medician Biotech Journal. 2014;3(2):59–68
  11. Hossain MA, Rahman SMM. Total phenolics, flavonoids and antioxidant activity of tropical fruit pineapple. Food Research International. 2011 Apr 1;44(3):672–6
  12. Indonesia D. Indonesia Pineapple Production up to 2.89 Million Tons in 2021 [Internet]. Dataindonesia.id. [cited 2023 Feb 16]. Available from: https://dataindonesia.id/sektor- riil/detail/indonesia-produk-nanas- anggaran-289-juta-ton-pada-2021
  13. Ali Ghasemzadeh. Flavonoids and phenolic acids: Role and biochemical activity in plants and humans. J Med Plants Res [Internet]. 2011 Dec 23
  14. [cited 2023 Feb 16];5(31). Available from: http://www.academicjournals.org/J MPR/abstracts/abstracts/abstracts20 11/23Dec/Ghasemzadeh%20and%2 0Ghasemzadeh.htm
  15. Mappa MR, Kuna MR, Akbar H. Utilization of Pineapple (Ananas comosus L.) as an antioxidant to increase body immunity in the era of the Covid 19 pandemic. Community Engagement and Emergence Journal (CEEJ). 2021 Dec 29;2(3):63–7
  16. Agarwal A, Sharma RK, Gupta S, Boitrelle F, Finelli R, Parekh N, et al. Sperm Vitality and Necrozoospermia: Diagnosis, Management, and Results of a Global Survey of Clinical Practice. World J Men's Health. 2022 Apr;40(2):228– 42
  17. el-Gothamy Z, el-Samahy M. Ultrastructure sperm defects inaddicts. Fertile Sterile. 1992 Mar;57(3):699–702
  18. Mahfouz RZ, du Plessis SS, Aziz N, Sharma R, Sabanegh E, Agarwal A. Sperm viability, apoptosis, and intracellular reactive oxygen species levels in human spermatozoa before and after induction of oxidative stress. Fertile Sterile. 2010 Feb;93(3):814–21
  19. Omolaoye TS, El Shahawy O, Skosana BT, Boillat T, Loney T, du Plessis SS. The mutagenic effect of tobacco smoke on male fertility. Environ Sci Pollut Res. 2022 Sep 1;29(41):62055–66
  20. Kim KH, Joo KJ, Park HJ, Kwon CH, Jang MH, Kim CJ. Nicotine induces apoptosis in TM3 mouse Leydig cells. Fertile Sterile. 2005 Apr;83 Suppl 1:1093–9
  21. Jana K, Samanta PK, De DK. Nicotine diminishes testicular gametogenesis, steroidogenesis, and steroidogenic acute regulatory protein expression in adult albino rats: possible influence on pituitary gonadotropins and alteration of testicular antioxidant status. Toxicol Sci. 2010 Aug;116(2):647–59
  22. Rusip G, Ilyas S, Lister INE, Ginting CN. The effect of ingestion of red dragon fruit extract on levels of malondialdehyde and superoxide dismutase after strenuous exercise in rats (Rattus norvegicus). F1000Res. 2021 Oct 18;10:1061
  23. Wibawa JC, Wati LH, Arifin MZ. Vitamin C Lowering MechanismOxidative Stress After Physical Activity. JOSSAE (Journal of Sport Science and Education). 2020 May 28;5(1):57–63

Last update:

No citation recorded.

Last update:

No citation recorded.