Effects of Andrographis paniculata Leaf Extract on C-Reactive Protein and Serum Ferritin in Lipopolysaccharide-Induced Sepsis Model Rat

Rezya Salsabela; Nyoman Suci Widyastiti; Dwi Retnoningrum; Ariosta Ariosta; Neni Susilaningsih

doi:10.14710/dmj.v12i3.37645

DOI: https://doi.org/10.14710/dmj.v12i3.37645

Effects of Andrographis paniculata Leaf Extract on C-Reactive Protein and Serum Ferritin in Lipopolysaccharide-Induced Sepsis Model Rat

Rezya Salsabela - Faculty of Medicine, Diponegoro University, Semarang, Indonesia

*Nyoman Suci Widyastiti

- Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Dwi Retnoningrum - Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Ariosta Ariosta - Department of Clinical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

Neni Susilaningsih - Department of Anatomy-Histology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia

BibTex Citation Data :

@article{dmj37645,
    author = {Rezya Salsabela and Nyoman Widyastiti and Dwi Retnoningrum and Ariosta Ariosta and Neni Susilaningsih},
    title = {Effects of Andrographis paniculata Leaf Extract on C-Reactive Protein and Serum Ferritin in Lipopolysaccharide-Induced Sepsis Model Rat},
    journal = {Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal)},
  volume = {12},
    number = {3},
    year = {2023},
    keywords = {Andrographis paniculata; CRP; serum ferritin; lipopolysaccharide; sepsis},
    abstract = {  Background:  C-reactive protein (CRP) and ferritin are acute-phase reactants that have demonstrated association with the severity of a variety of inflammation-mediated illnesses, including infection and sepsis. Sambiloto (Andrographis paniculata) leaf is traditionally used as an anti-inflammation and anti-bacterial herb. Sambiloto leaf extract possesses anti-inflammatory, antiallergenic, immuno-stimulatory, antiviral, and antioxidant activities   Objective:  To evaluate the effect of Sambiloto leaf extract (Andrographis paniculata) on inflammation biomarker (CRP and serum ferritin) in a rat model of sepsis-induced by lipopolysaccharide (LPS).   Methods:  This research was a true experimental study with a post-test-only control group design. Twenty five male Wistar rats were randomly divided into 5 groups consisting of a healthy control (HC), Negative control (NC), Treatment 1 (T1) Sambiloto 200mg/kgBW, T2 Sambiloto 400mg/kgBW, T3 Sambiloto 500mg/kgBW). Sambiloto leaf extract was given on days 8-21 and LPS 5mg/kgBW was injected intraperitoneal on day 22. On the 25th day, blood was drawn and  CRP and serum ferritin levels were analyzed using ELISA method. The data were analyzed using One-Way ANOVA and Kruskal Wallis test.   Results:  The mean CRP levels of HC, NC, T1, T2,T3 were 7.72 ± 2.53; 8.12 ± 1.08; 5.22 ± 1.71; 6.40 ± 1.19; 5.14 ± 1.37 ng/dl respectively. The mean serum ferritin level of HC, NC, T1, T2, T3 were 293.60 ± 66.53; 601.20 ± 100.17; 433.40 ± 194.65; 331.60 ± 75.97; 318.00 ± 69.64 ng/dl respectively. There were differences in CRP levels in groups T1(p=0.012) and T3(p=0.010), serum ferritin levels in groups T2(p=0.014) and T3(p=0.008) to the control group.  Conclusion:  Sambiloto (Andrographis paniculata) leaf extract  has anti-inflammatory effects in the rat model of sepsis.},
   issn = {2540-8844},   pages = {131--136}  doi = {10.14710/dmj.v12i3.37645},
    url = {https://ejournal3.undip.ac.id/index.php/medico/article/view/37645}
}

Citation Format:

Abstract

Background: C-reactive protein (CRP) and ferritin are acute-phase reactants that have demonstrated association with the severity of a variety of inflammation-mediated illnesses, including infection and sepsis. Sambiloto (Andrographis paniculata) leaf is traditionally used as an anti-inflammation and anti-bacterial herb. Sambiloto leaf extract possesses anti-inflammatory, antiallergenic, immuno-stimulatory, antiviral, and antioxidant activities

Objective: To evaluate the effect of Sambiloto leaf extract (Andrographis paniculata) on inflammation biomarker (CRP and serum ferritin) in a rat model of sepsis-induced by lipopolysaccharide (LPS).

Methods: This research was a true experimental study with a post-test-only control group design. Twenty five male Wistar rats were randomly divided into 5 groups consisting of a healthy control (HC), Negative control (NC), Treatment 1 (T1) Sambiloto 200mg/kgBW, T2 Sambiloto 400mg/kgBW, T3 Sambiloto 500mg/kgBW). Sambiloto leaf extract was given on days 8-21 and LPS 5mg/kgBW was injected intraperitoneal on day 22. On the 25th day, blood was drawn and CRP and serum ferritin levels were analyzed using ELISA method. The data were analyzed using One-Way ANOVA and Kruskal Wallis test.

Results: The mean CRP levels of HC, NC, T1, T2,T3 were 7.72 ± 2.53; 8.12 ± 1.08; 5.22 ± 1.71; 6.40 ± 1.19; 5.14 ± 1.37 ng/dl respectively. The mean serum ferritin level of HC, NC, T1, T2, T3 were 293.60 ± 66.53; 601.20 ± 100.17; 433.40 ± 194.65; 331.60 ± 75.97; 318.00 ± 69.64 ng/dl respectively. There were differences in CRP levels in groups T1(p=0.012) and T3(p=0.010), serum ferritin levels in groups T2(p=0.014) and T3(p=0.008) to the control group.

Conclusion: Sambiloto (Andrographis paniculata) leaf extract has anti-inflammatory effects in the rat model of sepsis.

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Keywords: Andrographis paniculata; CRP; serum ferritin; lipopolysaccharide; sepsis

Funding: This research is funded by Faculty of Medicine, Diponegoro University, grant No: 1664/UN7.5.4.2/PP2021)

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Section: Original Articles

Language : EN

In Vol 12, No 3 (2023): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)

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