DOI: https://doi.org/10.14710/dmj.v12i6.37311

The Effect of Moringa oleifera Extract on Histopathological of Mice Testes Exposed by Monosodium Glutamate

Regenio Akira Handoyo  -  Undergraduate Program, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia, Indonesia
Indah Saraswati  -  Department of Biology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia, Indonesia
Arufiadi Anityo Mochtar  -  Department of Obstetric and Gynecology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia, Indonesia
*Desy Armalina orcid scopus  -  Dept of Histology, Faculty of Medicine Universitas Diponegoro Semarang, Indonesia
Ika Pawitra Miranti  -  Department of Anatomical Pathology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia, Indonesia

Citation Format:
Abstract

Background: Monosodium glutamate (MSG) consumption in excess can have an impact on a man's fertility and lead to infertility. MSG has the potential to produce free radicals, which can harm cells. Antioxidants can fight off free radicals. Exogenous antioxidants are required to maintain equilibrium since there will be an imbalance if the body produces more free radicals than endogenous antioxidants. Exogenous antioxidants flavonoids are present in the leaves of the Moringa plant (Moringa oleifera L). It is thought that flavonoids have 4-5 times more antioxidant potential than vitamins. Objectives: To evaluate how moringa leaf extract affects the histopathological profile of MSG-exposed mouse testes. Methods: Only the posttest was used as a control group in this study. 25 male mice were used as the sample, and they were split up into five groups. The treatment group 1 (P1) received 6 g/day of MSG exposure + 300 mg/kg BW/day of moringa leaf extract, the treatment group 2 (P2) received 6 g/day of MSG exposure + 600 mg/kg BW/day of moringa leaf extract, and the treatment group 3 (P3) received 6 g/day of MSG exposure + 1200 mg/kg BW/day of moringa leaf extract. The control group (K-) received only standard feed. The mice were put to death after 30 days. The testes were removed for histological examination using hematoxylin-eosin staining, and the Johnsen scoring criteria were applied to their evaluation. Results: The data were not significant according to the Shapiro-Wilk test in the P3 group (MSG 6 g/day + Moringa Extract 1200 mg/kg BW/day). The Kruskal-Wallis test revealed that there was no significant difference between the seminiferous tubules according to the treatment group (p = 0.117). Conclusion: Moringa oleifera leaf extract protects the histopathological picture of the testes of male mice compared to the group given monosodium glutamate. 

Note: This article has supplementary file(s).

Fulltext View|Download |  Research Instrument
Untitled
Subject
Type Research Instrument
  Download (1MB)    Indexing metadata
 Research Instrument
ethical clearance
Subject
Type Research Instrument
  View (92KB)    Indexing metadata
Keywords: Testicular histopathology, Moringa oleifera leaf, Monosodium glutamate, Free radicals, Antioxidants

Article Metrics:

Article Info
Section: Original Articles
Language : EN
Recent articles
Correlation between Knowledge, Attitudes, and Behaviour of Mothers about Exclusive Breastfeeding and Its Success Rate in Karangmulyo Village Analysis of Comorbidities and Smoking History with The Incidence of Pulmonary Tuberculosis at Surakarta General Hospital The Effect of Moringa oleifera Extract on Histopathological of Mice Testes Exposed by Monosodium Glutamate Case Report : Chromoblastomycosis More recent articles
  1. REFERENCES
  2. Kurtanty D, Faqih DM, Upa NP. Review Monosodium Glutamat How to Understand it Properly? Vol. 4, Katalog dalam Penerbitan (KDT) Primer Koperasi Ikatan Dokter Indonesia. 2018
  3. Edward Z. Pengaruh Pemberian Monosodium Glutamat (MSG) Pada Tikus Jantan (Rattus norvergicus) Terhadap FSH dan LH. Maj Kedokt Andalas. 2010;34(2):160
  4. Budiman J, Istiadi H, Amarwati S. Pengaruh madu terhadap gambaran mikroskopis testis pada tikus wistar yang diinduksi monosodium glutamat. Media Med muda. 2015;4(4):723–31
  5. Winarsi H. Antioksidan Alami dan Radikal Bebas, Potensi dan Aplikasinya dalam Kesehatan [Internet]. 5th ed. Yogyakarta: KANISIUS (Anggota IKAPI); 2011. Available from: https://books.google.co.id/books?id=AlC1KQ2Oaj0C&printsec=copyright#v=onepage&q&f=false
  6. Arifin B, Ibrahim S. Struktur, Bioaktivitas Dan Antioksidan Flavonoid. J Zarah. 2018;6(1):21–9
  7. Parwata MOA. Bahan Ajar Antioksidan. Kim Terap. 2016;(April):1–54
  8. Munhoz VM, Longhini R, Souza JRP, Zequi JAC, Mello EVSL, Lopes GC, et al. Extraction of flavonoids from Tagetes patula: Process optimization and screening for biological activity. Brazilian J Pharmacogn. 2014;24(5):576–83
  9. A Dudi Krisnadi. KELOR, Super Nutrisi. Morindo. Pusat Informasi dan Pengembangan Tanaman Kelor Indonesia. 2015. 160 p
  10. Hardiyanthi F. Pemanfaatan Aktivitas Antioksidan Ekstrak Daun Kelor ( Moringa oleifera ) Dalam Sediaan Hand and Body Cream. Fak Sains dan Teknol UIN Syarif Hidayatullah. 2015;
  11. Gopalakrishnan L, Doriya K, Kumar DS. Moringa oleifera: A review on nutritive importance and its medicinal application. Food Sci Hum Wellness [Internet]. 2016;5(2):49–56. Available from: http://dx.doi.org/10.1016/j.fshw.2016.04.001
  12. Pratama Putra I, Dharmayudha A, Sudimartini L. Identifikasi Senyawa Kimia Ekstrak Etanol Daun Kelor (Moringa oleifera L) di Bali. Indones Med Veterinus. 2017;5(5):464–73
  13. Susantiningsih T. Obesitas dan Stress Oksidatif. J Biokimia, Fak Kedokteran, Univ Lampung. 2015;1
  14. Arief H, Widodo MA. Peranan Stres Oksidatif pada Proses Penyembuhan Luka. J Ilm Kedokt Wijaya Kusuma. 2018;5(2):22
  15. Christijanti W, Utami NR. Efek Pemberian Antioksidan Vitamin C dan E terhadap Kualitas Spermatozoa Tikus Putih Terpapar Allethrin. J Biol Biol Educ. 2011;2(1):18–26
  16. Sukmaningsih a. a. S., Ermayanti IGAM, Wiratmini NI, Sudatri NW. Gangguan Spermatogenesis Setelah Pemberian Monosodium Glutamat Pada Mencit( Mus musculus L .). J Biol. 2011;XV(2):49–52
  17. Das RS, Ghosh SK. Long term effects of monosodium glutamate on spermatogenesis following neonatal exposure in albino mice--a histological study. Nepal Med Coll J. 2010;12(3):149–53
  18. Daiber A, Di Lisa F, Oelze M, Kröller-Schön S, Steven S, Schulz E, et al. Crosstalk of mitochondria with NADPH oxidase via reactive oxygen and nitrogen species signalling and its role for vascular function. Br J Pharmacol. 2017;174(12):1670–89
  19. Stefani GP, Baldissera G, Nunes RB, Heck TG, Rhoden CR. Metabolic Syndrome and DNA Damage: The Interplay of Environmental and Lifestyle Factors in the Development of Metabolic Dysfunction. Open J Endocr Metab Dis. 2015;05(07):65–76
  20. Schieber M, Chandel NS. ROS function in redox signaling and oxidative stress. Curr Biol [Internet]. 2014;24(10):R453–62. Available from: http://dx.doi.org/10.1016/j.cub.2014.03.034
  21. Celino FT, Yamaguchi S, Miura C, Ohta T, Tozawa Y, Iwai T, et al. Tolerance of spermatogonia to oxidative stress is due to high levels of Zn and Cu/Zn superoxide dismutase. PLoS One. 2011;6(2):1–11
  22. Akunna G, Saalu L, Ogunlade B, Ogunmodede O, Akingbade A. Anti-Fertility Role of Allethrin Based-Mosquito Coil on Animal Models. Int J Biol Pharm Allied Sci. 2013;2(2):192–207
  23. Akunna G, Nwafor J, Egwu O A, Akingbade A M, Olasehinde O R, O FC, et al. Follicular counts and biochemical evaluation in ovary of Wistar Rats exposed to Esbiothrin-based mosquito repellant . SAS J Med [Internet]. 2017;3(6):103–11. Available from: http://sassociety.com/sasjm/
  24. Akingbade A M; Saalu L C; Oyebanji O O; Oyeniran D A; Akande O O; Akuma G G. Rhodinol-based Incense Testiculotoxicity in Albino Rats: Testicular Histology, Spermatogenic and Biochemical Evaluations. J Pharmacol Toxicol. 2014;2:68–81
  25. Eweka A, Eweka A, Om'Iniabohs F. Histological studies of the effects of monosodium glutamate on the fallopian tubes of adult female wistar rats. Ann Biomed Sci. 2010;2(3):146–9
  26. Bera TK, Kar SK, Yadav PK, Mukherjee P, Yadav S, Joshi B. Effects of monosodium glutamate on human health: A systematic review Tushar. World J Pharm Sci. 2017;5(May):139–44
  27. Husarova V, Ostatnikova D. Monosodium Glutamate Toxic Effects and Their Implications for Human Intake: A Review. JMED Res. 2013;(March 2014):1–12
  28. Jannah R, Setiasih NLE, Suastika P. Histopathological of Diabetes Mellitus White Rat Testicle After Given Moringa Leaf Extract. Bul Vet Udayana. 2018;10(2):176
  29. Kasolo JN, Bimenya GS, Ojok L, Ochieng J, Ogwal-Okeng JW. Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. J Med Plants Res. 2010;4(9):753–7
  30. Cahyani S L. Skrining Fitokimia dan Aktivitas Penangkapan Radikal Bebas DPPH Ekstrak Etanol Daun Kelor (Moringa Oleifera) di Ende. J Kedokt Udayana. 2017;410–6
  31. Baldwin L. Research Concepts for the Practitioner of Educational Leadership. The Library of Congress Cataloging in Publication Data. The Library of Congress Cataloging-in-Publication Data; 2018. 74 p
  32. Moodley I. Evaluation of Sub Chronic Toxicity of Moringa Oleifera Leaf Powder in Mice. J Toxicol Pharmacol Res Artic [Internet]. 2017; Available from: www.oecd.org/test/monos/htm]
  33. Widowati L, Winarno MW, Intan PR, Teknologi P, Kesehatan T. Toksisitas Akut dan Subkronis Ramuan Ekstrak Kelor dan Klabet sebagai Pelancar ASI dan Penambah Gizi Lucie. J Kefarmasian Indones. 2014;4(2):51–66
  34. Osman H., Shayoub M., Babiker E., Faiza A., Munzir ME., Bashier O, et al. Assessment of acute toxicity and LD 50 of Moringa oleifera ethanolic leave extract in albino rats and rabbits. J Med Biol Sci Res. 2015;1(4):38–43

Last update:

No citation recorded.

Last update:

No citation recorded.