COMPARATIVE STUDY OF LIVER HISTOPATHOLOGY AND TRANSAMINASE LEVELS IN DIABETIC WISTAR RATS INDUCED BY ALLOXAN AND STREPTOZOTOCIN

Ainun Nafisa Riski Hidayah; Ratna Damma Purnawati; Pulong Wijang Pralampita; Akhmad Ismail

doi:10.14710/dmj.v15i3.53470

DOI: https://doi.org/10.14710/dmj.v15i3.53470

COMPARATIVE STUDY OF LIVER HISTOPATHOLOGY AND TRANSAMINASE LEVELS IN DIABETIC WISTAR RATS INDUCED BY ALLOXAN AND STREPTOZOTOCIN

*Ainun Nafisa Riski Hidayah - Undergraduate Programme, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia, Indonesia

Ratna Damma Purnawati - Department of Histology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia, Indonesia

Pulong Wijang Pralampita - Department of Histology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia, Indonesia

Akhmad Ismail - Department of Histology, Faculty of Medicine, Universitas Diponegoro, Semarang, Indonesia, Indonesia

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

BibTex Citation Data :

@article{dmj53470,
    author = {Ainun Hidayah and Ratna Purnawati and Pulong Pralampita and Akhmad Ismail},
    title = {COMPARATIVE STUDY OF LIVER HISTOPATHOLOGY AND TRANSAMINASE LEVELS IN DIABETIC WISTAR RATS INDUCED BY ALLOXAN AND STREPTOZOTOCIN},
    journal = {Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal)},
  volume = {15},
    number = {3},
    year = {2026},
    keywords = {},
    abstract = {  Background:  Diabetes mellitus (DM) is a metabolic disorder characterized by elevated blood glucose levels. Experimental models using male Wistar rats (Rattus norvegicus) induced with alloxan (ALX) or streptozotocin (STZ) are widely applied to explore therapeutic strategies. However, several studies have reported that ALX has greater limitations compared with STZ.  Objective:  To analyze the differential effects of ALX and STZ as diabetogenic inducers in Wistar rats based on liver histopathology, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT) levels.  Methods:  A post-test only control group design with simple randomization was employed. Forty-two male Wistar rats weighing ≥200 g were assigned into seven treatment groups. Independent variables included ALX and STZ at graded doses, while dependent variables were liver histopathology, SGOT, and SGPT. Histopathology was assessed using the Manja Roenigk scoring system, and enzyme levels were measured by enzymatic methods.  Results:  Significant differences were observed between ALX and STZ in liver histopathology (p<0.001) and SGPT levels (p=0.001), but not in SGOT (p=0.199). Histopathological findings between ALX- and STZ-induced groups were relatively comparable at equivalent doses. In contrast, SGOT and SGPT results indicated that ALX may induce resistance.  Conclusion:  ALX and STZ caused comparable hepatic damage, but STZ exhibited more stable blood biomarker profiles. },
   issn = {2540-8844},   pages = {234--239}  doi = {10.14710/dmj.v15i3.53470},
    url = {https://ejournal3.undip.ac.id/index.php/medico/article/view/53470}
}

Citation Format:

Abstract

Background: Diabetes mellitus (DM) is a metabolic disorder characterized by elevated blood glucose levels. Experimental models using male Wistar rats (Rattus norvegicus) induced with alloxan (ALX) or streptozotocin (STZ) are widely applied to explore therapeutic strategies. However, several studies have reported that ALX has greater limitations compared with STZ. Objective: To analyze the differential effects of ALX and STZ as diabetogenic inducers in Wistar rats based on liver histopathology, serum glutamic oxaloacetic transaminase (SGOT), and serum glutamic pyruvic transaminase (SGPT) levels. Methods: A post-test only control group design with simple randomization was employed. Forty-two male Wistar rats weighing ≥200 g were assigned into seven treatment groups. Independent variables included ALX and STZ at graded doses, while dependent variables were liver histopathology, SGOT, and SGPT. Histopathology was assessed using the Manja Roenigk scoring system, and enzyme levels were measured by enzymatic methods. Results: Significant differences were observed between ALX and STZ in liver histopathology (p<0.001) and SGPT levels (p=0.001), but not in SGOT (p=0.199). Histopathological findings between ALX- and STZ-induced groups were relatively comparable at equivalent doses. In contrast, SGOT and SGPT results indicated that ALX may induce resistance. Conclusion: ALX and STZ caused comparable hepatic damage, but STZ exhibited more stable blood biomarker profiles.

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In Vol 15, No 3 (2026): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)

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