Histopathology of Seminiferous Tubules of Wistar Rats (Rattus norvegicus) Exposed to Electric Mosquito Repellent with Active Ingredient D-Allethrin

Salwa Nabila Izzati; Desy Armalina; RR. Mahayu Dewi Ariani; Fanti Saktini

doi:10.14710/dmj.v10i5.31588

DOI: https://doi.org/10.14710/dmj.v10i5.31588

Histopathology of Seminiferous Tubules of Wistar Rats (Rattus norvegicus) Exposed to Electric Mosquito Repellent with Active Ingredient D-Allethrin

Salwa Nabila Izzati - Medical student, Faculty of Medicine, Diponegoro University, Jl. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275, Indonesia

*Desy Armalina - Departement of Histology, Faculty of Medicine, Diponegoro University, Indonesia

RR. Mahayu Dewi Ariani

- Departement of Biology, Faculty of Medicine, Diponegoro University, Indonesia

Fanti Saktini

- Departement of Histology, Faculty of Medicine, Diponegoro University, Indonesia

BibTex Citation Data :

@article{dmj31588,
    author = {Salwa Izzati and Desy Armalina and RR. Mahayu Ariani and Fanti Saktini},
    title = {Histopathology of Seminiferous Tubules of Wistar Rats (Rattus norvegicus) Exposed to Electric Mosquito Repellent with Active Ingredient D-Allethrin},
    journal = {Jurnal Kedokteran Diponegoro (Diponegoro Medical Journal)},
  volume = {10},
    number = {5},
    year = {2021},
    keywords = {Electrical mosquito repellent; d-allethrin; oxidative stress; seminiferous tubules; spermatogenesis},
    abstract = {  Background:  An increase in mosquito-borne diseases has resulted in the use of mosquito repellents, especially electric ones in Indonesia. One of the active ingredients of electric mosquito repellent, namely  d-allethrin , cangenerate free radicals resulting in oxidative stress. Oxidative stress on the testes can interfere with the spermatogenesis stage in the seminiferous tubules.   Objective  :  Determine the histopathology of seminiferous tubules of Wistar rats exposed to electric mosquito repellents with the active ingredient  d-allethrin .   Method  s  :  This study used a true experimental design with a post test only control group design. A sample of 25 Wistar rats was divided into 5 groups. The control group (K) was given only standard feed. Treatment group 1 (P1), treatment group 2 (P2), treatment group 3 (P3), and treatment group 4 (P4) were exposed to electric mosquito repellents with active ingredient  d-allethrin  sequentially for 8 hours/day, 12 hours/day, 18 hours/day, and 24 hours/day. After 30 days the rats were terminated. The testes were taken for histopathological observation with HE staining and assessed according to the Johnsen score criteria.   Result  s  :  Based on the score, the mean value of the control group was 9.4, P1: 8.36, P2: namely 7.68, P3: 5.84, and P4: 5.64.The results of statistical tests using the one way Anova showed a significant difference (p <0.001).   Conclusion:  Exposure to electric mosquito repellent containing the active ingredient  d-allethrin  caused differences in the histopathology of seminiferous tubules score of Wistar rats. },
   issn = {2540-8844},   pages = {390--395}  doi = {10.14710/dmj.v10i5.31588},
    url = {https://ejournal3.undip.ac.id/index.php/medico/article/view/31588}
}

Citation Format:

Abstract

Background: An increase in mosquito-borne diseases has resulted in the use of mosquito repellents, especially electric ones in Indonesia. One of the active ingredients of electric mosquito repellent, namely d-allethrin, cangenerate free radicals resulting in oxidative stress. Oxidative stress on the testes can interfere with the spermatogenesis stage in the seminiferous tubules.

Objective: Determine the histopathology of seminiferous tubules of Wistar rats exposed to electric mosquito repellents with the active ingredient d-allethrin.

Methods: This study used a true experimental design with a post test only control group design. A sample of 25 Wistar rats was divided into 5 groups. The control group (K) was given only standard feed. Treatment group 1 (P1), treatment group 2 (P2), treatment group 3 (P3), and treatment group 4 (P4) were exposed to electric mosquito repellents with active ingredient d-allethrin sequentially for 8 hours/day, 12 hours/day, 18 hours/day, and 24 hours/day. After 30 days the rats were terminated. The testes were taken for histopathological observation with HE staining and assessed according to the Johnsen score criteria.

Results: Based on the score, the mean value of the control group was 9.4, P1: 8.36, P2: namely 7.68, P3: 5.84, and P4: 5.64.The results of statistical tests using the one way Anova showed a significant difference (p <0.001).

Conclusion: Exposure to electric mosquito repellent containing the active ingredient d-allethrin caused differences in the histopathology of seminiferous tubules score of Wistar rats.

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Keywords: Electrical mosquito repellent; d-allethrin; oxidative stress; seminiferous tubules; spermatogenesis

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Section: Original Articles

Language : EN

In Vol 10, No 5 (2021): JURNAL KEDOKTERAN DIPONEGORO (DIPONEGORO MEDICAL JOURNAL)

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