BibTex Citation Data :
@article{JMR38661, author = {Wilhelmina Sinulingga and Diah Wijayanti and Munasik Munasik and Dwi Haryanti}, title = {Analisa Genetika Gastropoda Nudibranchia dari Pulau Panjang, Jepara}, journal = {Journal of Marine Research}, volume = {13}, number = {3}, year = {2024}, keywords = {Nudibranchia; Keanekaragaman; DNA; Spesies}, abstract = { Gastropoda Nudibranchia memiliki karakteristik insang telanjang atau insang berbulu atau tanduk di punggungnya merupakan anggota Ordo Opisthobranchia, kelompok Gastropoda terbesar dengan anggota lebih dari 3.000 species. Nudibranchia diketahui hidup di Pulau Panjang, salah satu destinasi wisata terkenal di Jepara. Pergerakannya yang lambat dan bentuknya yang menarik, dapat mengganggu keberadaannya di habitat alaminya. Penelitian mengenai Nudibranchia belum banyak dilakukan di Indonesia, termasuk analisa genetiknya. Penelitian ini bertujuan untuk menganalisis keanekaragaman genetik Nudibranchia dari Perairan Pulau Panjang. Analisa DNA dilakukan di Laboratorium Terpadu Universitas Diponegoro, dengan metode PCR-sequencing menggunakan mtDNA-COI (cytochrome oxidase subunit I gene dari DNA mitokondrial). Primer yang digunakan pada penelitian yaitu primer forward: LCOI1490: 5'-GGTCAACAAATCATAAAGATATTGG-3 dan reverse: HCOI2198: 5'-TAAACTTCAGGGTGACC AAAAAATCA-3'. Rekonstruksi pohon filogenetik dan keragaman genetik dilakukan menggunakan software MEGA 6.06 (Analysis of the Evolution of Molecular Genetics). Hasil analisis sampel Nudibranchia berdasarkan susunan DNA mitokondria, ditemukan 2 spesies dengan 8 individu yaitu, spesies Jorunna funebris dan Chromodoris lineolata . Tingkat kesamaan (homologi) dalam analisa BLAST (Basic Local Alignment Search Tool) sebesar 98 % - 100 %. Hasil analisis filogenetik secara menyeluruh memperlihatkan pengelompokan yang terjadi berdasarkan kemiripan genetiknya. Nudibranchia gastropods have the characteristics of naked gills or hairy gills or horns on their backs and are members of the Order Opisthobranchia, the largest group of Gastropods with more than 3,000 species. Nudibranchia are known to live on Panjang Island, one of the famous tourist destinations in Jepara. Its slow movements and attractive shape can disrupt its existence in its natural habitat. Not much research has been done on Nudibranchia in Indonesia, including genetic analysis. This research aims to analyze the genetic diversity of Nudibranchia from Panjang Island waters. DNA analysis was carried out at the Diponegoro University Integrated Laboratory, using the PCR-sequencing method using mtDNA-COI (cytochrome oxidase subunit I gene from mitochondrial DNA). The primers used in the research were forward primer: LCOI1490: 5'-GGTCAACAAATCATAAAGATATTGG-3 and reverse: HCOI2198: 5'-TAAACTTCAGGGTGACCAAAAAATCA-3'. Phylogenetic tree reconstruction and genetic diversity were carried out using MEGA 6.06 (Analysis of the Evolution of Molecular Genetics) software. The results of analysis of Nudibranchia samples based on mitochondrial DNA structure found 2 species with 8 individuals, namely, Jorunna funebris and Chromodoris lineolata species. The level of similarity (homology) in BLAST (Basic Local Alignment Search Tool) analysis is 98% - 100%. The results of a comprehensive phylogenetic analysis show that grouping occurs based on genetic similarity. }, issn = {2407-7690}, pages = {547--554} doi = {10.14710/jmr.v13i3.38661}, url = {https://ejournal3.undip.ac.id/index.php/jmr/article/view/38661} }
Refworks Citation Data :
Gastropoda Nudibranchia memiliki karakteristik insang telanjang atau insang berbulu atau tanduk di punggungnya merupakan anggota Ordo Opisthobranchia, kelompok Gastropoda terbesar dengan anggota lebih dari 3.000 species. Nudibranchia diketahui hidup di Pulau Panjang, salah satu destinasi wisata terkenal di Jepara. Pergerakannya yang lambat dan bentuknya yang menarik, dapat mengganggu keberadaannya di habitat alaminya. Penelitian mengenai Nudibranchia belum banyak dilakukan di Indonesia, termasuk analisa genetiknya. Penelitian ini bertujuan untuk menganalisis keanekaragaman genetik Nudibranchia dari Perairan Pulau Panjang. Analisa DNA dilakukan di Laboratorium Terpadu Universitas Diponegoro, dengan metode PCR-sequencing menggunakan mtDNA-COI (cytochrome oxidase subunit I gene dari DNA mitokondrial). Primer yang digunakan pada penelitian yaitu primer forward: LCOI1490: 5'-GGTCAACAAATCATAAAGATATTGG-3 dan reverse: HCOI2198: 5'-TAAACTTCAGGGTGACC AAAAAATCA-3'. Rekonstruksi pohon filogenetik dan keragaman genetik dilakukan menggunakan software MEGA 6.06 (Analysis of the Evolution of Molecular Genetics). Hasil analisis sampel Nudibranchia berdasarkan susunan DNA mitokondria, ditemukan 2 spesies dengan 8 individu yaitu, spesies Jorunna funebris dan Chromodoris lineolata. Tingkat kesamaan (homologi) dalam analisa BLAST (Basic Local Alignment Search Tool) sebesar 98 % - 100 %. Hasil analisis filogenetik secara menyeluruh memperlihatkan pengelompokan yang terjadi berdasarkan kemiripan genetiknya.
Nudibranchia gastropods have the characteristics of naked gills or hairy gills or horns on their backs and are members of the Order Opisthobranchia, the largest group of Gastropods with more than 3,000 species. Nudibranchia are known to live on Panjang Island, one of the famous tourist destinations in Jepara. Its slow movements and attractive shape can disrupt its existence in its natural habitat. Not much research has been done on Nudibranchia in Indonesia, including genetic analysis. This research aims to analyze the genetic diversity of Nudibranchia from Panjang Island waters. DNA analysis was carried out at the Diponegoro University Integrated Laboratory, using the PCR-sequencing method using mtDNA-COI (cytochrome oxidase subunit I gene from mitochondrial DNA). The primers used in the research were forward primer: LCOI1490: 5'-GGTCAACAAATCATAAAGATATTGG-3 and reverse: HCOI2198: 5'-TAAACTTCAGGGTGACCAAAAAATCA-3'. Phylogenetic tree reconstruction and genetic diversity were carried out using MEGA 6.06 (Analysis of the Evolution of Molecular Genetics) software. The results of analysis of Nudibranchia samples based on mitochondrial DNA structure found 2 species with 8 individuals, namely, Jorunna funebris and Chromodoris lineolata species. The level of similarity (homology) in BLAST (Basic Local Alignment Search Tool) analysis is 98% - 100%. The results of a comprehensive phylogenetic analysis show that grouping occurs based on genetic similarity.
Article Metrics:
Last update:
Copyright Notice
Copyright for articles published in the Journal of Marine Research is held by the journal’s editorial management. The journal supports open access and the widespread dissemination of scholarly work.
Publishing Rights By submitting to this journal, authors grant the Journal of Marine Research the non-exclusive right to publish and distribute their work. All content is made freely available to the public and is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License (CC BY-SA 4.0). This license allows others to copy, redistribute, remix, transform, and build upon the material for any purpose, even commercially, as long as appropriate credit is given, a link to the license is provided, and any modified material is distributed under the same license.
