PENGARUH PEMBERIAN REKOMBINAN HORMON PERTUMBUHAN (rGH) MELALUI METODE PERENDAMAN DENGAN LAMA WAKTU YANG BERBEDA TERHADAP PERTUMBUHAN DAN KELULUSHIDUPAN IKAN LELE VARIETAS SANGKURIANG

Wisnu Hadi Triwinarso, Fajar Basuki, Tristiana Yuniarti

Abstract


Penelitian ini bertujuan untuk mengkaji pengaruh pertumbuhan benih ikan lele sangkuriang yang diberi rekombinan hormon pertumbuhan dosis 2mg/L dengan metode perendaman dengan lama waktu yang berbeda dan mengkaji waktu yang optimal benih ikan lele sangkuriang yang diberi rekombinan hormon pertumbuhan terhadap pertumbuhan dan kelangsungan hidup benih ikan lele sangkuriang. Penelitian ini dilaksanakan di Satker PBIAT Ngrajek, Magelang, pada bulan Agustus-November 2013. Penelitian menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan perendaman dengan larutan rekombinan hormon pertumbuhan 2mg/L perlakuan A selama 0 menit, perlakuan B 30 menit, perlakuan C 60 menit, dan perlakuan D 90 menit. rGH yang digunakan berasal dari ikan kerapu kertang (ElrGH). Hasil pengamatan pertumbuhan bobot spesifik perlakuan A sebesar 5,642±0,025 %/hari, perlakuan B sebesar 6,510±0,055 %/hari, perlakuan C sebesar 6,358±0,108 %/hari, perlakuan D sebesar 6,240±0,179 %/hari. Pengukuran panjang mutlak didapat hasil perlakuan A mendapatkan hasil 4,18±0,07 cm, pelakuan B 5,35 ± 0,03 cm, perlakuan C 5,30 ± 0,09 cm, dan perlakuan D 5,25 ± 0,23 cm. Nilai konversi pakan pada perlakuan A 0,751±0,008, perlakuan B 0,457±0,022, perlakuan C 0,514±0,010, perlakuan D 0,543±0,008. SR yang didapat selama pemeliharaan pada perlakuan A 82,67±1,53%, Perlakuan B 79,00±3,00% , perlakuan C 75,33±2,52%, perlakuan D 73,00±1,00%. Pemberian rekombinan hormon pertumbuhan melalui metode perendaman pada ikan lele sangkuriang dengan lama waktu 30 menit dapat meningkatkan pertumbuhan bobot spesifik, pertumbuhan panjang mutlak, dan kelulushidupan, serta menurunkan rasio konversi pakan. Pemberian rekombinan hormon pertumbuhan dapat meningkatkan SGR sebesar 15,90%, panjang mutlak 28%, kelulushidupan 13,25%, dan menurunkan FCR 64,33%

 

The purpose of this study was to assess the effect of seed growth sangkuriang catfish fed with recombinant growth hormone dose 2mg / L through immersion method with different time and assess the optimal time sangkuriang seed catfish fed recombinant growth hormone on growth and seed survival rate catfish sangkuriang. This study was conducted in Unit Freshwater Fish Hatchery Center (SATKER PBIAT) Ngrajek, Magelang, August-November 2013. Research using a completely randomized design (CRD) with 4 treatments and 3 replications. Immersion treatment with recombinant growth hormone solution of 2 mg / L treatment A for 0 min, 30 min treatment B, treatment of C 60 mins, and treatment D 90 minutes. The rGH used from giant grouper fish (rElGH). The observation of SGR treatment of A 5,642±0,025 %/day, treatment of B 6,510±0,055 %/day, treatment of C 6,358±0,108 %/day, treatment of D 6,240±0,179 %/day. The absolute length measurements that showed growth optimum results at A 4,18±0,07 cm, treatment of B 5,35 ± 0,03 cm, treatment of C 5,30 ± 0,09 cm, and treatment of D 5,25 ± 0,23 cm. Feed conversion value in treatment A 0,751±0,008, treatment B 0,457±0,022, treatment C 0,514±0,010, treatment D 0,543±0,008. SR obtained during the maintenance treatment A 82,67±1,53%, treatment B 79,00±3,00% , treatment C 75,33±2,52%, treatment D 73,00±1,00%. Administration of recombinant growth hormone through immersion method at catfish sangkuriang long 30 minutes can increase the growth of a specific weight, length of absolute growth, and survival, as well as lower feed conversion ratio. Administration of recombinant growth hormone can increase by 15.90% SGR, the absolute length of 28%, the survival of 13.25%, and 64.33% lower FCR


Keywords


Rekombinan hormon pertumbuhan; lele sangkuriang; perendaman

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