ANALISIS KANDUNGAN β-KAROTEN FUSAN INTRASPESIES Chlorella vulgaris dan APLIKASINYA SEBAGAI PAKAN TAMBAHAN PADA POST LARVA STADIA 10 UDANG WINDU (Penaeus monodon).

Abstract

Carotenoids are one of pigment which needed for development of post-larvae tiger shrimp (Penaeus monodon), but the shrimp unable to synthesis carotenoids by themselves in the body so it takes a feed containing carotenoids. Carotenoids can be obtained from synthetic feed or natural feed. One of the natural feed is microalgae Chlorella vulgaris. β-carotene is the highest content of carotenoid in C. vulgaris, this content can be improved by protoplast fusion. The purpose of this study was to determine the amount of β-carotene in fusan intraspecies C. vulgaris and it’s application as supplement in the post-larvae stadia 10^th tiger shrimp (P. monodon) Measurement of the amount of  β-carotene using UV-Vis at a wavelength of 460nm. The feeding is done three times a day. The results showed that the amount of β-carotene on fusan C. vulgaris is greater than the wildtype of C. vulgaris. β-carotene in the fusan C. vulgaris is14.88µg/g while the wildtype of C. vulgaris is 7.14 µg/g. Additional applications C. vulgaris by 10 x 10⁴ in 0.0225 g synthetys feed post-larvae stadia 10^th shrimp (P. monodon) compared to the post-larvae shrimp fed only control showed that the feed given additional fusan of C. vulgaris increases the weight of shrimp at 39.9%, while the given wildtype of C. vulgaris increasing shrimp weight by 10.1%.

Keywords: β-carotene, Chlorella vulgaris, protoplast fusion, post larvae shrimp