KERAGAMAN PROTEIN PLASMA DARAH KAMBING JAWARANDU DI KABUPATEN PEMALANG

Galih Dewa Brata, Sutopo Sutopo, Edy Kurnianto

Abstract


ABSTRACT

The aim of this study was to evaluate the variability of blood proteins plasma from Jawarandu goat at six loci by simultaneously. A total of 24 samples of blood protein plasm of Jawarandu goat from Pemalang District was taken. Vertical polyacrylamide gel electrophoresis system used to analyze blood plasma samples. The parameters observed were pre-albumin, albumin, ceruloplasmin, transferrin, post-transferrin and amylase-1 based interpretation bands appear from the results of electrophoresis. The results showed that each of 6 loci observed are controlled by two alleles. Individual heterozygosity values of each locus as follow pre-albumin (0.444), albumin (0.492), ceruloplasmin (0.500), transferrin (0.444), post-transferrin (0.486) and amylase-1 (0.486). The average heterozygosity of Jawarandu goat at Pemalang District was 0.475.

Keywords: genetic variability, jawarandu goat, heterozygosity

ABSTRAK

Penelitian ini bertujuan untuk melakukan evaluasi keragaman protein plasma darah dari kambing Jawarandu pada enam lokus secara simultan. Materi penelitian berupa 24 sampel plasma darah kambing Jawarandu yang terdapat di Kabupaten Pemalang. Teknik elektroforesis dengan gel poliakrilamid sistem vertikal digunakan untuk menganalisis sampel plasma darah. Parameter yang diamati meliputi pre-albumin, albumin, ceruloplasmin, transferin, post-transferin dan amylase-1 berdasarkan interpretasi pita-pita yang nampak dari hasil elektroforesis. Hasil penelitian menunjukkan bahwa 6 lokus tersebut masing-masing dikontrol oleh 2 alel. Nilai heterozigositas individual masing-masing lokus adalah pre-albumin (0,444), albumin (0,492), ceruloplasmin (0,500), transferin (0,444), post-transferin (0,486) dan amylase-1 (0,486). Rata-rata heterozigositas kambing Jawarandu di Kabupaten Pemalang sebesar 0,475.

Kata kunci : keragaman genetik, kambing Jawarandu, heterozigositas


Keywords


keragaman genetik; kambing Jawarandu; heterozigositas;genetic variability; jawarandu goat; heterozygosity

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